Hanna-instruments HI 901 Instrukcja Użytkownika

INSTRUCTION MANUALHI 901 / HI 902AUTOMATIC TITRATORNaOHTitrantConc.StandardizeElectrodeGeneralOptionsSelectMethodMethodOptionsBurettepH/mVATCEndpointV

2-4SETUP2.2 Safety MeasuresThe following safety measures must be followed:1. Always ensure that the power-supply cable is connected to a grounded main

7-10pH & mV MODE

8-1AUXILIARY FUNCTIONSChapter 8. Contents8 AUXILIARY FUNCTIONS ...8-38.1 Buret

8-2AUXILIARY FUNCTIONS

8-3AUXILIARY FUNCTIONS8.1 BuretteTo access the Burette screen, press from the main screen.Highlight the desired option and then press .8.1.1 Prime B

8-4AUXILIARY FUNCTIONSTo prime the burette, select Prime Burette from the Burette screen. Enter the number ofrinses and press .The number of burette

8-5AUXILIARY FUNCTIONSThe manual dispense volume must be between the limits shown bellow:0.001 to 4.500 mL for a 5 mL burette0.001 to 9.000 mL f

8-6AUXILIARY FUNCTIONS8.3 ResultsThe Data Parameters screen contains options regarding data parameters from the titrator,as follows:8.3.1 Review Last

8-7AUXILIARY FUNCTIONS8.3.2 Review Available ReportsUp to 100 reports can be recorded on the titrator. To view one of the recorded rep

8-8AUXILIARY FUNCTIONSOperator Name - Allows the operator name to be recorded in each report.Electrode Name - Allows the electrode name to be recorded

8-9AUXILIARY FUNCTIONS8.3.5 Logging Interval  pH/mVAllows the user to setup a time interval for auto-logging while in pH or mV mode (see 7.3Logging s

2-5SETUP2.3 Installation2.3.1 Titrator Front View2.3.2 Titrator Rear ViewNaOH0.1 MPositioning collarfor stirrer adjustmentLocking screwsinput

8-10AUXILIARY FUNCTIONS

9-1MAINTENANCE, PERIPHERALSChapter 9. Contents9 MAINTENANCE, PERIPHERALS ...9 - 39.1 Bure

9-2MAINTENANCE, PERIPHERALS

9-3MAINTENANCE, PERIPHERALS9 MAINTENANCE, PERIPHERALS9.1 Burette Maintenance9.1.1 Burette AssemblyThe included burette is delivered with a 25 mL syri

9-4MAINTENANCE, PERIPHERALS9.1.3 Disassembling BuretteTo dismount the dispensing tube and the aspiration tube follow these steps: Slide up the protec

9-5MAINTENANCE, PERIPHERALSTo attach the burette to the pump simply slide the burette into the pump support as shownin the figure presented in 9.1.2 B

9-6MAINTENANCE, PERIPHERALS Clean both the piston and the syringe with appropriate solvents, then with ethylicalcohol and then rinse them several tim

9-7MAINTENANCE, PERIPHERALS9.1.6 Burette Preparation (Titrant Filling)Before starting a titration, the burette must be properly filled with titrant in

9-8MAINTENANCE, PERIPHERALS9.2 Fuses ReplacementTo replace the fuses, follow these steps: Turn off the titrator. Remove the power cord from the pow

9-9MAINTENANCE, PERIPHERALS9.3.1 Connecting an External DisplayThe information shown on the titrator display can be viewed also on a Standard VGA disp

2-6SETUP2.3.3 Titrator Left-side View2.3.4 Titrator AssemblyNote: Assembly operations must be completed before connecting the titrator to the power-

9-10MAINTENANCE, PERIPHERALSThe correspondence between the Titrators Keypad and the United States 101-type externalkeyboard are:External PC Keyboard(

9-11MAINTENANCE, PERIPHERALS9.3.3 Connecting a PrinterA variety of parallel printers can be connected to the parallel port of the titrator using

9-12MAINTENANCE, PERIPHERALSWarning:The titrator and the external PC must be both turned OFF from the main switchbefore making the connection.Connect

A1-1APPENDIX 1Appendix 1. ContentsA1 TECHNICAL SPECIFICATIONS ... A1-3

A1-2APPENDIX 1

A1-3APPENDIX 1A1 TECHNICAL SPECIFICATIONSmV Range - 2000.0 to 2000.0 mVAccuracy ±0.1 mVResolution 0.1 mVpH Range - 2.000 to 20.000 pHAccuracy ±0.001

A1-4APPENDIX 1Titer Determination.Fixed mV or pH End Point Detection.Single Equivalence Point Detection, with the 1st or 2nd Derivatives of the titra

A2-1APPENDIX 2Appendix 2. ContentsA2 BACK TITRATION (HI 902 only) ...A2-3A2.1 Applicabili

A2-2APPENDIX 2

A2-3APPENDIX 2A2 BACK TITRATION (HI 902 only)A2.1 Applicability DomainBack titrations are generally used for one of these reasons: Reaction kinetics

2-7SETUP2.3.4.2 Attaching StirrerTo attach the stirrer to titrator, follow these steps: Attach the propeller (2) to the stirrer (1) by pressing it on

A2-4APPENDIX 2and back titrate the remaining acid with NaOH to determine the amount of CaCO3 present.2HCl excess + 2NaOH > 2NaCl + 2H2OIn order to

A2-5APPENDIX 2A2.3.3 Preparing the SampleFor this analysis can use commercial antacid pills. Crush a few pills in a mortar and separate a quantity we

A2-6APPENDIX 2 Slide the stirrer assembly up. Put the beaker on a hotplate. Heat gently until all the effervescence has ceased, then boil it for 1-

A3-1APPENDIX 3Appendix 3. ContentsA3 MULTIPLE EQUIVALENCE POINTS (HI 902 only) ...A3-3A3.1 Applicability Domain ...

A3-2APPENDIX 3

A3-3APPENDIX 3A3 MULTIPLE EQUIVALENCE POINTS (HI 902 only)A3.1 Applicability DomainIn case of: determining the components concentration from a synth

A3-4APPENDIX 3A3.3 Titration ExampleAs an example of a multi-equivalence point titration we take the titration of a mixturecontaining HCl,

A3-5APPENDIX 3 The graph displays the equivalence points with a cross. Near each cross theequivalence titrant volume is diplayed. The volu

A3-6APPENDIX 3

A4-1APPENDIX 4Appendix 4. ContentsA4 ACCESSORIES ...A4-3A4.1 Solution

2-8SETUP2.3.4.3 Connecting the PumpTo connect the pump, follow these steps: Retrieve the pump cable from inside the bay. The pump 1 connector is loca

A4-2APPENDIX 4

A4-3APPENDIX 4A4 ACCESSORIESA4.1 SolutionsA4.1.1 pH Calibration SolutionsHI 70004C > pH 4.01 Buffer Solution, 20 mLHI 77400C > pH 4.01 & 7

A4-4APPENDIX 4A4.1.4 Electrode Cleaning Solutions in FDA Approved BottleHI 8061M > General Purpose Solution, 230 mLHI 8061L > General Purpose

A4-5APPENDIX 4A4.2 SensorsA4.2.1 pH ElectrodesHI 1043B / HI 1040SGlass-body, double junction, refillable, combination pH electrode.Use: strong acid/a

A4-6APPENDIX 4FC 210BGlass-body, double junction, conic, Viscolene, combination pH electrode.Use: milk, yogurt.FC 220BGlass-body, single junction, ref

A4-7APPENDIX 4A4.2.4 Extension Cable for Screw-Type Electrodes only (Screw to BNC Connector)HI 7855/1Extension cable 1m (3.3') long.A4.2.5Tem

A4-8APPENDIX 4A4.3 Titrator componentsPump assemblyBurette (25 mL syringe)Aspiration tube with fittingand protection tubeDispensing tube with normald

A4-9APPENDIX 4Shorting capInstruction Manual BinderStart-up Disk (FDD withTitrator Installation Kit)HI 901 / 902HI901/902HI 900 Series Titration Sy

A4-10APPENDIX 4MAN901/90209/04 R0

2-9SETUPHI 902 only:Follow the previous steps (2.3.4.3) to connect and attach the second pump.2.3.4.4 Attaching Burette Blank Support (HI 901 only)The

2-10SETUP2.3.4.6 Electrical Connections Connect the pH electrode to the BNC connector (5). Connect the temperature sensor to the RCA connector (6).

2-11SETUP2.3.5 Connection to EarthIt is important that the titrator has a good connection to Earth. The titrator is properlygrounded

2-12SETUP

3-1USER INTERFACEChapter 3. Contents3 USER INTERFACE ... 3-33.1 Star

3-2USER INTERFACE

3-3USER INTERFACE3 USER INTERFACE3.1 Start UpOnce the instrument is assembled and installed, please follow these steps to start up thetitrator: Conne

3-4USER INTERFACE3.2 DescriptionThis chapter describes the basic principles of navigating through the user interface, selectingfields and entering val

3-5USER INTERFACE3.2.1.3 Arrow KeysThese keys have the following functions: Move the on-screen cursor. Increase and decrease the stirrer speed and o

3-6USER INTERFACEThe user interface contains several screens. For each titrator function, one or more screensare used.Warnings and other critical info

3-7USER INTERFACE3.3 Menu navigation3.3.1 Selecting an OptionTo select an option, simply press the option key belowthe virtual option key. For e

3-8USER INTERFACE3.3.4 Saving ModificationsThe Saving Method screen allows the user to savethe modifications. To exit from Saving Method screenw

4-1GENERAL OPTIONSChapter 4. Contents4 GENERAL OPTIONS ...4-34.1 Active

4-2GENERAL OPTIONS

4-3GENERAL OPTIONS4 GENERAL OPTIONSThe General Options screen gives access to options that are not directly related to the titrationprocess or pH / mV

TABLE OF CONTENTSChapter 1. INTRODUCTIONChapter 2. SETUPChapter 3. USER INTERFACEChapter 4. GENERAL OPTIONSChapter 5. METHODSChapter 6. TITRATION MODE

4-4GENERAL OPTIONS Highlight the pump to be activated. Press and the highlighted pump is set as temporarily active (for the durationof the current

4-5GENERAL OPTIONS4.2.1 Temperature SourceThe Temperature Source screen allows the user to select the temperature source, usedfor pH temperature co

4-6GENERAL OPTIONS4.2.3 Temperature UnitsThe following temperature units can be selected.The temperature ranges are as displayed in the Temperature Un

4-7GENERAL OPTIONSOption Keys:Turns the backlight offTurns the backlight onIncreases the backlight saver time intervalDecreases the backlight saver ti

4-8GENERAL OPTIONS4.5 BeeperThis screen allows the beeper to be enabled (Beeper On) or disabled (Beeper Off).The beeper will sound after a titration i

4-9GENERAL OPTIONS4.7 LanguageSelect the language from the available languages listed in this screen.4.8 Daily Standardization ReminderThis screen a

4-10GENERAL OPTIONS4.9 Periodic Standardization ReminderThis screen allows a programmable reminder to appear when it is time to standardize the pHel

4-11GENERAL OPTIONSAfter the new titrant volume has been set on titrator (in the Total Volume Alert screen), awarning message appears reminding the us

4-12GENERAL OPTIONSThe option keys allow the following operations:Deletes the highlighted fileDeletes all currently displayed filesCopies the highligh

4-13GENERAL OPTIONSThe file types that can be transferred are:Standard Method Files - HI*.MTDUser Method Files - USER*.MTDReport Files - *.RPTSelecti

Dear customer,Thank you for choosing a Hanna Instruments Product.This instruction manual has been written for the HI 901 / HI 902 Titrator products.Pl

4-14GENERAL OPTIONS4.15 Reset to Default SettingsThis option restores the manufacturer settings.Note: Please be careful !!! This will also delete all

5-1METHODSChapter 5. Contents5 METHODS ...5 - 35.1 Sele

5-2METHODS5.5.11 Calculation ...5 - 275.5.11.1 Stand

5-3METHODS5 METHODSThe titrator automatically performs a complete analysis.A complete analysis comprises sample preparation, dispensing of titrant sol

5-4METHODSTo select a method, highlight the method and press . The name of the selectedmethod will be displayed on the main screen.5.2 S

5-5METHODS5.2.2 Deleting Standard MethodsYou can remove unnecessary standard methods from titrator following the next steps: Insert any diskette into

5-6METHODS5.3.1 Creating User MethodsTo create a new user method start from a standard or user method and follow these steps: Press from the main s

5-7METHODS5.4 View / Modify MethodTo modify the methods parameters, press from the main screen. A list of all theparameters for the selected

5-8METHODS5.4 Method Options5.5.1 Naming the User MethodThis option allows you to enter a name for the new method (up to 24 characters). Use thearrow

5-9METHODSBack TitrationA titration with a pH or mV equivalence point detection, consisting of two titration phases: Phase 1  the sample is consume

1-1INTRODUCTION1 INTRODUCTIONHI 901 and HI 902 are automatic titrators with high accuracy, great flexibility and repeatability.The titrators are desig

5-10METHODS5.5.3.1 Linear DosingThis type of dosing uses equal volume doses of titrant as shown in the graph below.The Linear Dosing option is re

5-11METHODSThe allowed ranges for dosing volume are:0.001 to 4.500 mL for a 5 mL burette0.001 to 9.000 mL for a 10 mL burette0.005 to 22.500

5-12METHODSAs a result, far from the end point, larger doses are made, reducing the total titration time.Closer to the end point, smaller doses are ma

5-13METHODS5.5.4 End Point Mode5.5.4.1 Fixed End Point (pH or mv)Fixed End Point (pH):The titration is normally terminated when the preset pH

5-14METHODSFixed End Point (mV):The end point detection algorithm is the same as for pH, but the threshold value is expressedin mV.The range is from -

5-15METHODSNumber of Equivalence Points (HI 902 only)The titrator can perform a titration with more than one equivalence point.Up to 5 equivalence poi

5-16METHODSThis is rigorously exact only for symmetric S-shaped titration curves. For non-symmetric curves,the effect of this theoretical error ca

5-17METHODS2nd Derivative:When second derivative is used to recognize the equivalence point, the zero crossing point ofthe second derivative correspon

5-18METHODSThreshold:This parameter must be set by the user in according with the analysis.The threshold represents the absolute value of the first de

5-19METHODSRange:Range is an optional feature for equivalence point recognition. It represents a set mV or pHrange for equivalence point detection.The

1-2INTRODUCTION

5-20METHODSFiltered Derivatives:Filtered Derivatives is an optional feature for equivalence point recognition.This option adds a filtering procedure i

5-21METHODSThe valid pre-titration volume is between the ranges shown below:0.001 to 4.500 mL for a 5 mL burette0.001 to 9.000 mL for a 10 mL bu

5-22METHODS5.5.7 Measurement ModeDuring titration, the acquisition of the potential (mV) value of the solution, can be performedin two ways, by using

5-23METHODSThe signal stability window (condition) represents the time interval ∆t during which thepotential measured in solution (mV)

5-24METHODS5.5.7.2 Timed IncrementWhen Timed Increment option is selected, the acquiring of the potential (mV) value from thesolution is made at a fix

5-25METHODS5.5.8 Electrode TypeEnter the type of the electrode, up to 24 characters. The electrode type will appear in thetitration report.5.5.9 Blank

5-26METHODSIf one of the options (V-Blank or Blank-V ) is selected in the View / Modify Method screenthe Blank Value option will became active and the

5-27METHODSThe imposed current can be set from -100 µA to 100 µA with 1 µA resolution.5.5.11 CalculationsThe titrator will make certain calculations i

5-28METHODSSample Calculations by WeightThis calculation should be used when the concentration of an analyte is determined in a solidsample. The resul

5-29METHODSIf the titrant unit is selected as M (mol/L) and the final sample unit as g/Kg (grams/kilogram)the formula used to generate the result is d

2-1SETUPChapter 2. Contents2 SETUP ... 2-32.1 Unpack

5-30METHODSM (mol/L) (Molarity; moles/liter)N (eq/L) (Normality; equivalences/liter)mg/L (milligrams/liter)µg/L (micrograms/liter)mmol/L (millimoles/l

5-31METHODSThe titrator will provide the calculation based on the titrant unit selected.Standardize Titrant by VolumeThis calculation should be used w

5-32METHODSeq/L (equivalences/liter)meq/L (milliequivalences/liter)The formula can be used either for titrant standardization or for sample analysis.

5-33METHODSTitrant Concentration:The units for titrant concentration can be:mol/L (concentration unit of titrant)eq/L (concentration unit of titrant)

5-34METHODS5.5.11.2 Back Titrations (HI 902 only)Sample Calculations by WeightWhen choosing this formula, select the titrant 1 unit, the titrant 2 uni

5-35METHODSIf the titrant 1 unit is selected as M (mol/L), titrant 2 unit is selected as M (mol/L) and thefinal result unit as mg/g (milligrams/gram)

5-36METHODSIn order to calculate this volume, the remaining titrant 1 volume is titrated (pH/mV equivalencepoint titration) with titrant 2.After the e

5-37METHODS⇐ Select the titrant 2 unit⇐ Select the final result unitThe titrator will provide the calculation based on the titrants and sample units s

5-38METHODSThis calculation formula is used as an approximation of the sample concentration (samplemax conc.). This formula can be used when we have a

5-39METHODSWhen all the parameters are set, press to proceed with the Calculating SampleConcentration formula:Generic FormulaThis opti

2-2SETUP

5-40METHODSThe sample size used in the calculations will be:Analyte size to be diluted * Aliquot VolumeFinal Dilution Volume5.5.13 Titrant NameEnter t

5-41METHODS5.5.16.1 Fixed Weight or VolumeEach titration will use the same analyte weight or volume as preset in the calculations.5.5.16.2 Manual W

5-42METHODS5.5.18 Maximum Titrant VolumeThe maximum titrant volume used in the titration must be set according to the analysis.If the titration end po

5-43METHODS5.5.20 Potential RangeThe allowable input potential range can be set by the user. Outside of these limits, if thetitration is in progress,

5-44METHODS5.5.22 Signal AveragingThis option enables or disables a filtering on the mV/pH reading.If 1 Reading is selected, the filtering is disabled

6-1TITRATION MODEChapter 6. Contents6 TITRATION MODE ...6-36.1 Titrat

6-2TITRATION MODE

6-3TITRATION MODE6 TITRATION MODE6.1 Titration StartBefore beginning to perform a titration make sure that the following conditions are met: A pump

6-4TITRATION MODEThe S-shaped curve and the derivative curve are scaled to fit simultaneously inside the display.Also, when the titration is normally

6-5TITRATION MODE - allows you to save the graph as a bitmap file. Available only when the titration isfinished (after end point detection).6.2 Titrat

2-3SETUP2 SETUP2.1 UnpackingThe titrator and the accessories are shipped in a single box containing:ITEM QUANTITY1 Titrator...

6-6TITRATION MODE

7-1pH & mV MODEChapter 7. Contents7 pH & mV MODE ...7-

7-2pH & mV MODE

7-3pH & mV MODE7 pH & mV MODEThe HI 901 / HI 902 titrators can be used to log precise pH or mV measurements.By pressing from the main screen

7-4pH & mV MODE7.1.2 pH MenuOptions from this screen (except the Resolution option) refer to the pH electrode calibrationfunctions.7.1.2.1 Add a B

7-5pH & mV MODE7.1.2.3 Clear StandardizationDeletes electrode standardization. The titrator will prompt for confirmation.7.1.2.4 Edit Buffer SetC

7-6pH & mV MODE7.1.2.5 Graph Standardization DataGraphs the electrode standardization data (pH vs mV).7.1.2.6 pH Standardization DataDisplays th

7-7pH & mV MODE7.2.2 mV MenuBy pressing , the following screen will be displayed:7.2.2.1 Auto-Zero Relative mVThis sets the relative potential

7-8pH & mV MODE7.2.2.3 Clear Relative mV OffsetSets the mV offset to zero. With this option, the previous relative value or auto zero is cleared.7

7-9pH & mV MODE7.3.1 Automatic LoggingTo start automatic logging pH or mV readings, follow these steps: From the pH or mV screen, press to disp